RBS
Part:BBa_K2541116:Design
Designed by: Shuting Zheng Group: iGEM18_Jilin_China (2018-10-05)
Heat-repressible RNA-based thermosensor-16
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 21
Design Notes
The secondary structure is important to the function of an RNA-based thermosensor. We designed the thermosensor according to the stem length, loop size and mismatches or bulges in the stem.
Source
This sequence is not derived from any organism. We designed this sequence on our own and synthesized this sequence from a synthesis company.
References
[1]Hoynes-O'Connor A, Hinman K, Kirchner L, et al. De novo design of heat-repressible RNA thermosensors in E. coli[J]. Nucleic Acids Research, 2015, 43(12):6166-6179.
[2]Kortmann J, Narberhaus F. Bacterial RNA thermometers: molecular zippers and switches.[J]. Nature Reviews Microbiology, 2012, 10(4):255-65.